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Polymerase chain reaction PCR - Animation

By: HWC, Views: 1592

Polymerase chain reaction (PCR) is a method that amplifies fragments of DNA. The purpose of PCR is to create copies of a specific region of DNA. To use this technique, researchers must know the base sequences at either end of the region of interest. They use this information to create...

Semidiscontinuous DNA replication

By: HWC, Views: 7334

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...

Protein catabolism - deamination

By: HWC, Views: 7876

• Digestion hydrolyzes proteins into amino acids, which are transported to the bloodstream. • Amino acids and proteins are not stored. • Instead, they are: • Oxidized to generate ATP. • Used to synthesize new proteins. • Converted to carbohydrates or lipids for storage (if e...

The Lagging Strand in DNA Replication and Replication in Action

By: HWC, Views: 7216

The lagging strand is the strand of nascent DNA whose direction of synthesis is opposite to the direction of the growing replication fork. DNA backbones run in opposite directions, the strands in a DNA molecule are oriented antiparallel to one another. New DNA is made by enzymes called DNA...

Krebs cycle : Formation of acetyl coenzyme A and Electron transport chain

By: HWC, Views: 7808

The oxidation of glucose to produce ATP is cellular respiration. Four sets of reactions are involved: Glycolysis Formation of acetyl coenzyme A Krebs cycle reactions Electron transport chain reactions • The second pathway of glucose catabolism, formation of acetyl coenzyme A, is a transi...

The Lac Operon in E. Coli

By: Administrator, Views: 11787

The lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is no...

ribosome

By: HWC, Views: 7463

The trp operon in E. coli contains five structural genes corresponding to enzymes that convert chorismate into tryptophan. The trpE gene contains a 5' untranslated region that plays an important role in the regulatory mechanism called attenuation. The 5' UTR contains four regions. Region 1 en...

Protein catabolism (Krebs cycle) and Protein anabolism (protein synthesis)

By: HWC, Views: 8169

• Deaminated acids are brought into the Krebs cycle to be oxidized to CO2 and H2O. • Before entering the Krebs cycle, the deaminated acids are converted into intermediate products (pyruvic acid, acetyl coenzyme A, carbonic acids). • In the Krebs cycle, amino acids are oxidized to form r...

Lipid catabolism ( ketogenesis and oxidation of glycerol) and Lipid anabolism (lipogenesis)

By: HWC, Views: 8028

• During excessive beta oxidation, the two-carbon fatty acid fragments are converted into acidic ketone bodies. • Ketosis, the overproduction of ketone bodies, can lead to acidosis (ketoacidosis) of the blood. • After lipolysis, glycerol is converted to pyruvic acid. • Pyruvic aci...