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Michaelis–Menten equation & Kinetic parameters

By: HWC, Views: 7498

The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrat...

Pores and Ion Channels

By: HWC, Views: 6907

Transmembrane channels, also called membrane channels, are pores within a lipid bilayer. The channels can be formed by protein complexes that run across the membrane or by peptides. They may cross the cell membrane, connecting the cytosol, or cytoplasm, to the extracellular matrix. Membrane po...

Secondary and tertiary levels of protein structure Animation

By: HWC, Views: 1635

Amino acid sequence dictates a protein's final shape. The presence of certain amino acids favors a pattern of hydrogen bonding that causes part of the polypeptide chain to coil and twist into an alpha helix. The presence of other amino acids enables hydrogen bonding between strand like re...

Power Supply Polyacrylamide Gel Protein Sample

By: HWC, Views: 7082

SDS-polyacrylamide gel electrophoresis is a powerful tool, which resolves proteins according to their molecular weights. Because proteins differ in size, shape, and charge, a protein sample is first denatured with the anionic detergent SDS. When the sample is heated, the SDS molecules bind to ...

Type of Cell Junctions - Desmosome, Hemidesmosomes and Gap Junctions

By: HWC, Views: 8073

Cell Junctions: Cell junctions are found in some multi-cellular organisms. They exist of complexes and are found between cells and between cells and other structures. The junctions provide a way for cells to connect and exchange signals. What are tight junctions, desmosomes, and gap junctions...

Protein digestion - stomach & small intestine

By: HWC, Views: 7193

• Protein digestion occurs in the stomach and small intestine. • The stomach enzyme pepsin initiates the process. • Pancreatic and intestinal brush border enzymes complete the digestive process. • In the stomach, pepsin is created from pepsinogen in the presence of pH-lowering hyd...

Transcription—A molecular view

By: HWC, Views: 3313

Transcription, as related to genomics, is the process of making an RNA copy of a gene's DNA sequence. This copy, called messenger RNA (mRNA), carries the gene's protein information encoded in DNA. During transcription, a DNA molecule is copied into RNA molecules that are then used to translate...

Proteins

By: HWC, Views: 6770

Living things must accomplish a great number of tasks just to get through a day, and these tasks are accomplished by a diverse range of biological molecules. In the range of tasks that molecules accomplish, however, proteins reign supreme. Almost every chemical reaction that takes place in living...

Hershey Chase Experiments

By: HWC, Views: 4886

Hershey and Chase knew that T4 bacteriophages consist of proteins and DNA. They asked which viral component must enter a bacterial cell to infect it: DNA or protein? They grew viruses with either radioactive sulfur, which labels the viral protein, or radioactive phosphorus, which labels DNA. ...