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Polymerase chain reaction PCR - Animation

By: HWC, Views: 1615

Polymerase chain reaction (PCR) is a method that amplifies fragments of DNA. The purpose of PCR is to create copies of a specific region of DNA. To use this technique, researchers must know the base sequences at either end of the region of interest. They use this information to create...

DNA close up Animation

By: HWC, Views: 1665

A section from a DNA double helix The backbone of each DNA strand consists of alternating deoxyribose sugars and phosphate groups. The two strands run in opposite directions. One runs from the 5' to 3' direction, the other in the 3' to 5' direction. Think of the deoxyribose units o...

Subunits of DNA And Semi Conservative Replication

By: HWC, Views: 4065

Adenine is a purine with a double-ring structure. In double-stranded DNA, adenine base-pairs with thymine. Guanine is a purine with a double-ring structure. In double-stranded DNA, guanine base-pairs with cytosine. Thymine is a pyrimidine with a single-ring structure. In double-stranded DNA, th...

Major Elements in Biological Molecules: Nucleic acids

By: HWC, Views: 7638

DNA and RNA are nucleic acids (polymers of nucleotides). Two polymers with complementary nucleotide sequences can pair with each other. This pairing endows nucleic acids with the ability to store, transmit, and retrieve genetic information. Two strands of DNA pair by hydrogen bonding. A compon...

New DNA Strand Replication

By: HWC, Views: 7135

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...

SNP Polymorphysim Microarray Chip - How to Test a Person's DNA

By: HWC, Views: 7044

To test a person's DNA, a researcher first needs a source of tissue. Most of the cells in a blood sample are red blood cells, which lack nuclei, but there are also a number of white blood cells, which do contain nuclei and chromosomal DNA. If we could see a particular DNA sequence in these cel...

The Lagging Strand in DNA Replication and Replication in Action

By: HWC, Views: 7231

The lagging strand is the strand of nascent DNA whose direction of synthesis is opposite to the direction of the growing replication fork. DNA backbones run in opposite directions, the strands in a DNA molecule are oriented antiparallel to one another. New DNA is made by enzymes called DNA...

DNA Replication Factory and Protein

By: HWC, Views: 7247

DNA (deoxyribose nucleic acid) carries all the genetic information needed to re-create itself and to pass on the characteristics of the organism. The “factory” model of DNA replication hypothesizes a specific nuclear structure in which the molecular machinery for replication forks are brou...

Semidiscontinuous DNA replication

By: HWC, Views: 7351

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...