Search results for: 'helicase enzyme'

Watch Later 3:50

By: HWC, Views: 7119

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...

Watch Later 3:03

Semidiscontinuous DNA replication

By: HWC, Views: 7335

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...

Watch Later 1:43

DNA Replication Factory and Protein

By: HWC, Views: 7231

DNA (deoxyribose nucleic acid) carries all the genetic information needed to re-create itself and to pass on the characteristics of the organism. The “factory” model of DNA replication hypothesizes a specific nuclear structure in which the molecular machinery for replication forks are brou...

Watch Later 2:24

Enzyme-Catalyzed Reactions

By: HWC, Views: 7180

S P Substrate in great excess ([S] -- [E]) (More correctly [S] -- KM, but more on this later) Zero order rate equation: v = k where k is the zero-order rate constant (with respect to substrate). (More correctly [S] -- KM, but more on this later) Substrate forms complex with enzyme. ...

Watch Later 2:42

Virtual Enzyme Kinetics & Lineweaver Burk Plot

By: HWC, Views: 7299

• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive...

Watch Later 1:03

Mechanism of enzyme action

By: HWC, Views: 7592

■ The substrate makes contact with the active site. ■ A change in the shape of the enzyme occurs resulting in the transformation of the substrate. ■ A substrate may undergo decomposition, have its atoms rearranged, or two substrates may be synthesized. ■ The products of the reaction...

Watch Later 1:59

Michaelis–Menten equation & Kinetic parameters

By: HWC, Views: 7496

The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrat...

Watch Later 3:27

Elementary kinetics

By: HWC, Views: 7014

✔ https://HomeworkClinic.com ✔ https://Videos.HomeworkClinic.com ✔ Ask questions here: https://HomeworkClinic.com/Ask Follow us: ▶ Facebook: https://www.facebook.com/HomeworkClinic ▶ Review Us: https://trustpilot.com/review/homeworkclinic.com Kinetics is the study of the rat...

Watch Later 0:57

Effect of the environment on coat color in the Himalayan rabbit Animation

By: HWC, Views: 3239

An organism's phenotype—the combination of traits that we observe—is the product of interactions between its genotype and the environment. For example. a Himalayan rabbit is completely white at birth. But within weeks, the fur on the rabbits ears, nose, tail. and lower legs darkens. The...

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