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Hierarchical Sequencing Method - Sequence Tagged Sites

By: HWC, Views: 6961

In the hierarchical sequencing method, researchers begin by collecting cells. In humans, each cell contains 23 pairs of chromo-somes. Here we specifically track the DNA from just one of the 23 pairs. Chromosomes have a series of unique DNA sequences, called sequence-tagged sites (STSs), that a...

Mechanisms of capillary exchange (transcytosis & bulk flow)

By: HWC, Views: 7398

■ This method of capillary exchange is mainly used to transport small amounts of large, lipid-insoluble (water soluble) molecules, such as large proteins. ■ Substances, packaged in vesicles, move through endothelial cells via endocytosis and exocytosis. ■ This method of exchange is th...

Mechanisms of capillary exchange

By: HWC, Views: 7876

■ The primary role of capillaries is to permit the exchange of nutrients and wastes between the blood and tissue cells (via interstitial fluid). ■ Oxygen and nutrients move from the blood to the cells. ■ Carbon dioxide and other wastes move from the cells to the blood. The three ba...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7042

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Transferring genes into plants Animation

By: HWC, Views: 5077

Researchers extract DNA from an organism that has a trait they want to introduce into a plant. The genetic donor can be a bacterial cell, a plant cell. or even an animal cell. The desired gene will be transferred into a plasmid, a small circle of bacterial DNA. The gene is cut out of th...

Labor and Delivery - Vacuum Extraction

By: Administrator, Views: 375

Vacuum extraction (VE), also known as ventouse, is a method to assist delivery of a baby using a vacuum device. It is used in the second stage of labor if it has not progressed adequately. It may be an alternative to a forceps delivery and caesarean section. It cannot be used when the baby is in ...

Labor and Delivery - Postpartum Assessment

By: Administrator, Views: 474

The postpartum patient's emotional status plays a significant part in her recovery and her adjustment to her infant. Postpartum hospital stays are very brief, so nurses must make every encounter with the patient meaningful. Developing a systematic method of assessing the patient will save time an...

Exocytosis - secretion

By: HWC, Views: 7738

Exocytosis is another method of vesicular transport that moves large volumes Of fluid or chemicals out of the cell. It is a process by which a cell transports secretory products through the cytoplasm to the plasma membrane. A examples of cellular secretory products: 1. Secreted protein - enzym...

Polymerase chain reaction PCR - Animation

By: HWC, Views: 1593

Polymerase chain reaction (PCR) is a method that amplifies fragments of DNA. The purpose of PCR is to create copies of a specific region of DNA. To use this technique, researchers must know the base sequences at either end of the region of interest. They use this information to create...