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DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7043

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Replication of DNA and Chromosomes/ How do cells replicate their DNA? (Animation) no Audio

By: HWC, Views: 7462

DNA replication in E. coil begins at a site called oriC where a replication bubble forms. At either end of this bubble is a replication fork. Since DNA polymerase Ill can read its DNA template strand only in the 3' to 5' direction this means that one strand (leading) can be read continuously b...

Semidiscontinuous DNA replication

By: HWC, Views: 7339

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...

DNA Replication

By: HWC, Views: 4588

DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell.

The Lagging Strand in DNA Replication and Replication in Action

By: HWC, Views: 7217

The lagging strand is the strand of nascent DNA whose direction of synthesis is opposite to the direction of the growing replication fork. DNA backbones run in opposite directions, the strands in a DNA molecule are oriented antiparallel to one another. New DNA is made by enzymes called DNA...