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Power Supply Polyacrylamide Gel Protein Sample

By: HWC, Views: 7110

SDS-polyacrylamide gel electrophoresis is a powerful tool, which resolves proteins according to their molecular weights. Because proteins differ in size, shape, and charge, a protein sample is first denatured with the anionic detergent SDS. When the sample is heated, the SDS molecules bind to ...

Protein digestion - stomach & small intestine

By: HWC, Views: 7209

• Protein digestion occurs in the stomach and small intestine. • The stomach enzyme pepsin initiates the process. • Pancreatic and intestinal brush border enzymes complete the digestive process. • In the stomach, pepsin is created from pepsinogen in the presence of pH-lowering hyd...

Transcription—A molecular view

By: HWC, Views: 3331

Transcription, as related to genomics, is the process of making an RNA copy of a gene's DNA sequence. This copy, called messenger RNA (mRNA), carries the gene's protein information encoded in DNA. During transcription, a DNA molecule is copied into RNA molecules that are then used to translate...

Proteins

By: HWC, Views: 6791

Living things must accomplish a great number of tasks just to get through a day, and these tasks are accomplished by a diverse range of biological molecules. In the range of tasks that molecules accomplish, however, proteins reign supreme. Almost every chemical reaction that takes place in living...

Hershey Chase Experiments

By: HWC, Views: 4907

Hershey and Chase knew that T4 bacteriophages consist of proteins and DNA. They asked which viral component must enter a bacterial cell to infect it: DNA or protein? They grew viruses with either radioactive sulfur, which labels the viral protein, or radioactive phosphorus, which labels DNA. ...

Peptide Bond Formation Animation

By: HWC, Views: 1558

During protein synthesis, peptide bonds link amino acids together in the order specified by DNA instructions. In this case, the first two amino acids in the protein are methionine and alanine. Here are ball-and-stick models of these amino acids. Peptide bond formation is a type of condensatio...

How do the different types of chromatography work? (No Audio)

By: HWC, Views: 7068

Chromatography is a term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through some type of porous matrix. A glass column is filled with beads of an inert matrix. The mixture of proteins to be purified is dissolved in a solution and passed ...

Major Elements in Biological Molecules: Proteins

By: HWC, Views: 7157

Proteins are chains of amino acids linked by peptide bonds. The 20 different amino acids used to make all proteins differ only in their side chains, and the properties of these side chains account for the great diversity of protein structure and function. Collagen is an example of how a prote...

Protein Structure - Primary, Secondary, Tertiary and Quaternary

By: HWC, Views: 7675

A protein's first order structure, or primary structure, begins with the amino acid sequence of the polypeptide chain. The 20 different amino acids can be arranged in an infinite number of sequences. For example, the hormone insulin, which regulates the uptake of glucose from the blood into ce...