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Virtual Enzyme Kinetics & Lineweaver Burk Plot

By: HWC, Views: 7323

• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive...

Major Elements in Biological Molecules: Lipids

By: HWC, Views: 7070

A triglyceride (also called triacylglycerol) is composed of three fatty acid molecules and one glycerol molecule. The fatty acids attach to the glycerol molecule by a covalent ester bond. The long hydrocarbon chain of each fatty acid makes the triglyceride molecule nonpolar and hydrophobic. Pa...

DNA close up Animation

By: HWC, Views: 1676

A section from a DNA double helix The backbone of each DNA strand consists of alternating deoxyribose sugars and phosphate groups. The two strands run in opposite directions. One runs from the 5' to 3' direction, the other in the 3' to 5' direction. Think of the deoxyribose units o...

Kinetic parameters & Kinetic experiment

By: HWC, Views: 7602

Kinetics is a measure of the speed or rate of a chemical reaction. A study of kinetics allows us to determine which variables to control (temperature, reactants, catalysts) and how to vary them in order to maximize the amount of products formed and minimize the time involved. Vmax = maximum ve...

What Are Carbohydrates? Importance of Carbs & High Carb Food

By: HWC, Views: 7838

We hear a lot about carbohydrates in the news. Everybody seems to be on a low-carb diet. The news media often has stories on this diet fad, and companies are busy producing products with reduced carbohydrates. What's this fascination with carbohydrates? In a word: "Diet." The fact is that carb...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7068

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

How to make cDNA Animation

By: HWC, Views: 2301

This animation shows how an mRNA transcript can be used to make a cDNA strand.

Polymerase chain reaction PCR - Animation

By: HWC, Views: 1623

Polymerase chain reaction (PCR) is a method that amplifies fragments of DNA. The purpose of PCR is to create copies of a specific region of DNA. To use this technique, researchers must know the base sequences at either end of the region of interest. They use this information to create...

Non-polar compounds - insolubility

By: HWC, Views: 7860

• A non-polar molecule has uniform distribution of electrons. • Non-polar compounds like fatty acids in lipids have a high proportion of carbon and hydrogen. • Lipids possess no charge or partial charge. • Lipids are not attracted to water molecules. • Lipids are not soluble in...