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Membrane Protein and Facilitated Transport (Passive Vs Active)

By: HWC, Views: 7365

Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into several broad categories depending on their location. Integral membrane proteins span the membrane, with hydrophobic amino acids interacting with the lipid bilayer and hy...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7067

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Molecules, Membrane Permeability and Structure

By: HWC, Views: 7209

Organisms are not isolated system at equilibrium and need to intake nutrients and electrolytes as remove wastes. Similarly Cells within an organism must also exchange compound by passing them through membrane. The permeability of a membrane is the rate of passive diffusion of molecules th...

New DNA Strand Replication

By: HWC, Views: 7147

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...