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DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7043

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Osmosis - Isotonic, Hypotonic, and Hypertonic​ Solutions

By: HWC, Views: 7824

Isotonic: Equal Water moves in and out of the cell at an equal rate. The cell remains unchanged. Hypotonic: "hypo" hippo Water moves into the cell, making it swell and get fat (like a hippo). Eventually the cell can rupture and burst (aka lyse). Hypertonic: "like a raisin" Water leaves...

Metabolic Rate, Heat and Thermoregulation - response to heat and cold stresses

By: HWC, Views: 7940

• A neuron group in the anterior portion of the hypothalamus controls heat balance. • Neurons in the preoptic region of the hypothalamus integrate signals that come from thermoreceptors. • The temperature control center in the preoptic region propagates control signals to two other part...

Virtual Enzyme Kinetics & Lineweaver Burk Plot

By: HWC, Views: 7300

• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive...

Cellular Respiration & Glucose Mobilization (Glucose transport & Phosphorylation of Glucose)

By: HWC, Views: 7522

Glucose is completely broken down into CO2 and H2O during the process of cellular respiration, which includes 3 stages: 1) glycolysis; 2) the Krebs Cycle; and 3) the electron transport chain. Glucose enters this energy yielding pathway of cellular respiration in the first stage known as...

Major Elements in Biological Molecules: Carbohydrates

By: HWC, Views: 7266

Carbohydrates include simple sugars (monosaccharides) as well as large polymers (polysaccharides). Glucose is a hexose, a sugar composed of six carbon atoms, usually found in ring form. A starch macromolecule is a polysaccharide composed of thousands of glucose units. Glucose molecules can be ...

ATPase function - membrane transport, contractile proteins and synthesis

By: HWC, Views: 8090

• Energy from ATP is used to move ions across the cell membrane during active transport. • This membrane protein transports sodium out of the cell and potassium into the cell. As such, it is called a sodium-potassium pump. • Because this pump also acts as an enzyme to hydrolyze ATP it i...

Mitochondrial Structure & ETC Protein Complexes (Protein Complexes and Electron Transport)

By: HWC, Views: 7337

The energy carrying molecules, NADH and FADH2, that were generated in glycolysis and the Krebs cycle, now are processed in the mitochondria where their high energy electrons are deposited in an electron chain complex located in the inner mitochondrial membranes. These high-energy electrons now dr...

Role of the respiratory system - effect of altered ventilation rates

By: HWC, Views: 7582

• Dissociation of the chemical substances in the body fluids can result in the production of free hydrogen ions. • The pH scale is used to measure the concentration of hydrogen ions in solution. • Normal blood pH values vary around 7.4. • When hydrogen ion concentration increases, t...