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Second Messengers in the Inositol-lipid Signaling Pathway

By: HWC, Views: 7014

Extracellular signals produce specific responses in target cells through the action of intracellular second messengers. Here, we focus on three second messengers, IP3, DAG, and Ca2+, all involved in the inositol-lipid signaling pathway. A hormone-receptor signal on the cell surface leads to the a...

Power Supply Polyacrylamide Gel Protein Sample

By: HWC, Views: 7110

SDS-polyacrylamide gel electrophoresis is a powerful tool, which resolves proteins according to their molecular weights. Because proteins differ in size, shape, and charge, a protein sample is first denatured with the anionic detergent SDS. When the sample is heated, the SDS molecules bind to ...

SNP Polymorphysim Microarray Chip - How to Test a Person's DNA

By: HWC, Views: 7051

To test a person's DNA, a researcher first needs a source of tissue. Most of the cells in a blood sample are red blood cells, which lack nuclei, but there are also a number of white blood cells, which do contain nuclei and chromosomal DNA. If we could see a particular DNA sequence in these cel...

The Hypothalamus: The Body's Thermostat (Human Thermostat)

By: HWC, Views: 6974

Normal body function requires a relatively constant body temperature, which is regulated by the body's thermostat, a region of the brain called the hypothalamus. The hypothalamus generates a temperature set point for the body and appears to be the major site for the integration of temperature inf...

How Hemoglobin Picks Up and Delivers Oxygen

By: HWC, Views: 7046

All of the cells in our bodies require oxygen (02) for survival and must release carbon dioxide (CO2) as a waste product. The respiratory and circulatory systems work together as delivery systems for these gases. The lungs exchange these gases between the environment and the bloodstream. The bloo...

Pasteur's Experiment

By: HWC, Views: 7122

Louis Pasteur designed a procedure to test whether sterile nutrient broth could spontaneously generate microbial life. To do this, he set up two experiments. In both, Pasteur added nutrient broth to flasks, bent the necks of the flasks into S shapes, and then boiled the broth to kill any existing...

The life cycle of protein

By: HWC, Views: 7380

The life cycle of a typical protein begins with its synthesis on a ribosome. As the polypeptide chain grows, molecules of a chaperone protein bind along its length. This prevents misfolding of the nascent polypeptide. ATP binding causes chaperone release. For most proteins, the polypeptide th...

ribosome

By: HWC, Views: 7482

The trp operon in E. coli contains five structural genes corresponding to enzymes that convert chorismate into tryptophan. The trpE gene contains a 5' untranslated region that plays an important role in the regulatory mechanism called attenuation. The 5' UTR contains four regions. Region 1 en...

Four Giant Convection Cells oF Earth

By: HWC, Views: 7059

Earth Has Four Giant Convection Cells Earth's climates range from dry and cold at the poles to wet and warm at the equator. These climates are dictated largely by the amount of solar radiation that a region receives. Solar radiation warms the Earth and the surrounding air, setting up convection c...