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Hierarchical Sequencing Method - Sequence Tagged Sites

By: HWC, Views: 5872

In the hierarchical sequencing method, researchers begin by collecting cells. In humans, each cell contains 23 pairs of chromo-somes. Here we specifically track the DNA from just one of the 23 pairs. Chromosomes have a series of unique DNA sequences, called sequence-tagged sites (STSs), that a...

Michaelis–Menten equation & Kinetic parameters

By: HWC, Views: 6400

The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrat...

How do the different types of chromatography work? (No Audio)

By: HWC, Views: 5989

Chromatography is a term for a variety of techniques in which a mixture of dissolved components is fractionated as it moves through some type of porous matrix. A glass column is filled with beads of an inert matrix. The mixture of proteins to be purified is dissolved in a solution and passed ...

Chromosome structural organization/ Mechanisms for chromosome movement Animation

By: HWC, Views: 2804

How the chromosome is organized. At metaphase, the chromosomes are duplicated and are at their most condensed. In each chromosome, two identical sister chromatids are held together at a constricted region called the centromere. When a chromosome is condensed, interactions among chromosomal ...

Plant Defense Mechanisms from Pathogens

By: HWC, Views: 6103

Plants and pathogens have coevolved such that pathogens can recognize plants by the sugars, or other molecules, they produce. Plants, in turn, can recognize pathogens by the molecules they produce. The ability to recognize pathogens allows plants to activate defense systems that can prevent wides...

How proteins function? How do proteins work?

By: HWC, Views: 6280

How proteins function is really about how proteins "do work" in cells. How do proteins work? Let's start thinking about protein function by looking at something important to you: your hair. Keratin is a structural protein that is composed of 2 intertwined or helical strands. Keratin is also f...

Protein catabolism (Krebs cycle) and Protein anabolism (protein synthesis)

By: HWC, Views: 7040

• Deaminated acids are brought into the Krebs cycle to be oxidized to CO2 and H2O. • Before entering the Krebs cycle, the deaminated acids are converted into intermediate products (pyruvic acid, acetyl coenzyme A, carbonic acids). • In the Krebs cycle, amino acids are oxidized to form r...