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Virtual Enzyme Kinetics & Lineweaver Burk Plot

By: HWC, Views: 7295

• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive...

Elementary kinetics

By: HWC, Views: 7012

✔ https://HomeworkClinic.com ✔ https://Videos.HomeworkClinic.com ✔ Ask questions here: https://HomeworkClinic.com/Ask Follow us: ▶ Facebook: https://www.facebook.com/HomeworkClinic ▶ Review Us: https://trustpilot.com/review/homeworkclinic.com Kinetics is the study of the rat...

Kinetic parameters & Kinetic experiment

By: HWC, Views: 7572

Kinetics is a measure of the speed or rate of a chemical reaction. A study of kinetics allows us to determine which variables to control (temperature, reactants, catalysts) and how to vary them in order to maximize the amount of products formed and minimize the time involved. Vmax = maximum ve...

Enzyme-Catalyzed Reactions

By: HWC, Views: 7180

S P Substrate in great excess ([S] -- [E]) (More correctly [S] -- KM, but more on this later) Zero order rate equation: v = k where k is the zero-order rate constant (with respect to substrate). (More correctly [S] -- KM, but more on this later) Substrate forms complex with enzyme. ...

Mechanism of enzyme action

By: HWC, Views: 7589

■ The substrate makes contact with the active site. ■ A change in the shape of the enzyme occurs resulting in the transformation of the substrate. ■ A substrate may undergo decomposition, have its atoms rearranged, or two substrates may be synthesized. ■ The products of the reaction...

Michaelis–Menten equation & Kinetic parameters

By: HWC, Views: 7495

The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrat...

Effect of the environment on coat color in the Himalayan rabbit Animation

By: HWC, Views: 3237

An organism's phenotype—the combination of traits that we observe—is the product of interactions between its genotype and the environment. For example. a Himalayan rabbit is completely white at birth. But within weeks, the fur on the rabbits ears, nose, tail. and lower legs darkens. The...

Enzyme structure - Properties of enzymes

By: HWC, Views: 7676

■ Enzymes are proteins that catalyze reactions. ■ Some enzymes have two parts: a protein or apoenzyme and a non-protein or cofactor. ■ Cofactor can be a metal ion or another organic molecule called a coenzyme. ■ Coenzymes often come from vitamins. ■ Cofactors affect the shape of...

New DNA Strand Replication

By: HWC, Views: 7119

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...