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Results for: 'enzyme'

Enzyme-Catalyzed Reactions

By: HWC, Views: 739

S P Substrate in great excess ([S] -- [E]) (More correctly [S] -- KM, but more on this later) Zero order rate equation: v = k where k is the zero-order rate constant (with respect to substrate). (More correctly [S] -- KM, but more on this later) Substrate forms complex with enzyme. ...

Virtual Enzyme Kinetics & Lineweaver Burk Plot

By: HWC, Views: 695

• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive...

Mechanism of enzyme action

By: HWC, Views: 1147

■ The substrate makes contact with the active site. ■ A change in the shape of the enzyme occurs resulting in the transformation of the substrate. ■ A substrate may undergo decomposition, have its atoms rearranged, or two substrates may be synthesized. ■ The products of the reaction...

Michaelis–Menten equation & Kinetic parameters

By: HWC, Views: 855

The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction. This equation relates the initial reaction rate (v0), the maximum reaction rate (Vmax), and the initial substrate concentration [S] through the Michaelis constant KM—a measure of the substrat...

Elementary kinetics

By: HWC, Views: 700

✔ ✔ ✔ Ask questions here: Follow us: ▶ Facebook: ▶ Review Us: Kinetics is the study of the rat...

Enzyme structure - Properties of enzymes

By: HWC, Views: 1092

■ Enzymes are proteins that catalyze reactions. ■ Some enzymes have two parts: a protein or apoenzyme and a non-protein or cofactor. ■ Cofactor can be a metal ion or another organic molecule called a coenzyme. ■ Coenzymes often come from vitamins. ■ Cofactors affect the shape of...

New DNA Strand Replication

By: HWC, Views: 639

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...

Semidiscontinuous DNA replication

By: HWC, Views: 580

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...

Protein digestion - stomach & small intestine

By: HWC, Views: 763

• Protein digestion occurs in the stomach and small intestine. • The stomach enzyme pepsin initiates the process. • Pancreatic and intestinal brush border enzymes complete the digestive process. • In the stomach, pepsin is created from pepsinogen in the presence of pH-lowering hyd...