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SNP Polymorphysim Microarray Chip - How to Test a Person's DNA

By: HWC, Views: 7044

To test a person's DNA, a researcher first needs a source of tissue. Most of the cells in a blood sample are red blood cells, which lack nuclei, but there are also a number of white blood cells, which do contain nuclei and chromosomal DNA. If we could see a particular DNA sequence in these cel...

Mutations and translation

By: HWC, Views: 4260

A mutation, which may arise during replication and/or recombination, is a permanent change in the nucleotide sequence of DNA. Damaged DNA can be mutated either by substitution, deletion or insertion of base pairs. Mutations, for the most part, are harmless except when they lead to cell death or t...

Major Elements in Biological Molecules: Nucleic acids

By: HWC, Views: 7638

DNA and RNA are nucleic acids (polymers of nucleotides). Two polymers with complementary nucleotide sequences can pair with each other. This pairing endows nucleic acids with the ability to store, transmit, and retrieve genetic information. Two strands of DNA pair by hydrogen bonding. A compon...

Nucleic acid digestion - brush border enzymes, end products & transport mechanism

By: HWC, Views: 7526

• Further digestion occurs at the microvilli (brush border) of the epithelial cells of the villi in the small intestine. • Two brush border enzymes complete nucleic acid digestion: • Phosphatases, which catalyze the cleavage of a phosphate to form a nucleoside (nitrogenous base and pent...

Subunits of DNA And Semi Conservative Replication

By: HWC, Views: 4065

Adenine is a purine with a double-ring structure. In double-stranded DNA, adenine base-pairs with thymine. Guanine is a purine with a double-ring structure. In double-stranded DNA, guanine base-pairs with cytosine. Thymine is a pyrimidine with a single-ring structure. In double-stranded DNA, th...

Semidiscontinuous DNA replication

By: HWC, Views: 7351

During DNA replication, one of the two DNA strands, the leading strand, is replicated continuously, or all at once, in the 5' to 3' direction. The other strand, called the lagging strand, is replicated discontinuously, or in pieces, in the 3' to 5' direction. This is necessary because DNA poly...

The 4 steps of translation Animation

By: HWC, Views: 3558

Translation is the process of formation of a polypeptide chain according to codon present in mRNA. The four steps of translation are: Activation or charging of tRNA Initiation – recognition of start codon, binding of ribosomal subunits to mRNA and formation of initiation complex with Met-tR...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7057

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Molecules, Membrane Permeability and Structure

By: HWC, Views: 7201

Organisms are not isolated system at equilibrium and need to intake nutrients and electrolytes as remove wastes. Similarly Cells within an organism must also exchange compound by passing them through membrane. The permeability of a membrane is the rate of passive diffusion of molecules th...