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A Human Karyotype Preparation Animation

By: HWC, Views: 4902

Blood is collected from the person being analyzed. The blood is added to a growth medium that also contains a chemical that stimulates mitosis. The cells are allowed to grow in this medium for two or three days at body temperature. Colchicine is added to arrest cell division at metaphase. T...

The Pressure Flow Model in a Plant

By: HWC, Views: 7121

The vascular system of plants has two transport tissues, called xylem and phloem. Xylem transports water and minerals, while phloem transports a variety of dissolved substances, including sugars and amino acids, throughout the plant. Water in the xylem always moves up, in the direction from th...

Chemical Buffers - protein buffer, phosphate buffer system and bicarbonate buffer system

By: HWC, Views: 7970

• There are a variety of chemicals in body fluids that prevent the fluids from undergoing large changes in. • These chemicals buffer or regulate fluctuations in H+ concentration. • Chemical buffers: • Bind to H+ ions when there are too many in a solution so pH remains normal. •...

Normal and abnormal blood pH

By: HWC, Views: 7796

• Dissociation of the chemical substances in the body fluids can result in the production of free hydrogen ions. • The pH scale is used to measure the concentration of hydrogen ions in solution. • Normal blood pH values vary around 7.4. • When hydrogen ion concentration increases, t...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7063

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Introduction to Photolithography - WC, BLF, PA, Hard and Soft baking

By: HWC, Views: 7342

Prior to use, wafers are chemically cleaned to remove particulate matter on the surface as well as any traces of organic, ionic, and metallic impurities. Barrier Layer Formation After cleaning, the silicon wafer is covered with the material which will serve as a barrier layer. The most commo...

Pores and Ion Channels

By: HWC, Views: 6924

Transmembrane channels, also called membrane channels, are pores within a lipid bilayer. The channels can be formed by protein complexes that run across the membrane or by peptides. They may cross the cell membrane, connecting the cytosol, or cytoplasm, to the extracellular matrix. Membrane po...

Mechanisms of capillary exchange (transcytosis & bulk flow)

By: HWC, Views: 7409

■ This method of capillary exchange is mainly used to transport small amounts of large, lipid-insoluble (water soluble) molecules, such as large proteins. ■ Substances, packaged in vesicles, move through endothelial cells via endocytosis and exocytosis. ■ This method of exchange is th...

New DNA Strand Replication

By: HWC, Views: 7145

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...