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Stages of Mitosis - Prophase, Prometaphase, Metaphase, Anaphase & Telophase

By: HWC, Views: 7449

In mitosis, the nucleus divides to produce two nuclei that are genetically identical to each other and to the parent nucleus. To prepare for division, the DNA replicates in the preceding interphase. Although the chromosomes are not yet compacted and visible as discrete bodies, we illustrate them ...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7067

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Hierarchical Sequencing Method - Sequence Tagged Sites

By: HWC, Views: 6988

In the hierarchical sequencing method, researchers begin by collecting cells. In humans, each cell contains 23 pairs of chromo-somes. Here we specifically track the DNA from just one of the 23 pairs. Chromosomes have a series of unique DNA sequences, called sequence-tagged sites (STSs), that a...

Plant Defense Mechanisms from Pathogens

By: HWC, Views: 7200

Plants and pathogens have coevolved such that pathogens can recognize plants by the sugars, or other molecules, they produce. Plants, in turn, can recognize pathogens by the molecules they produce. The ability to recognize pathogens allows plants to activate defense systems that can prevent wides...

Phases of an Action Potential - Resting Potential, Threshold, Rising, Falling, & Recovery Phases

By: HWC, Views: 7157

In this tutorial, we will review the phases of an action potential measured from a small area of a neuron's membrane. The action potential can be divided into five phases: the resting potential, threshold, the rising phase, the falling phase, and the recovery phase. When the neuron is at rest,...

Second Messengers in the Inositol-lipid Signaling Pathway

By: HWC, Views: 7017

Extracellular signals produce specific responses in target cells through the action of intracellular second messengers. Here, we focus on three second messengers, IP3, DAG, and Ca2+, all involved in the inositol-lipid signaling pathway. A hormone-receptor signal on the cell surface leads to the a...

Power Supply Polyacrylamide Gel Protein Sample

By: HWC, Views: 7111

SDS-polyacrylamide gel electrophoresis is a powerful tool, which resolves proteins according to their molecular weights. Because proteins differ in size, shape, and charge, a protein sample is first denatured with the anionic detergent SDS. When the sample is heated, the SDS molecules bind to ...

SNP Polymorphysim Microarray Chip - How to Test a Person's DNA

By: HWC, Views: 7053

To test a person's DNA, a researcher first needs a source of tissue. Most of the cells in a blood sample are red blood cells, which lack nuclei, but there are also a number of white blood cells, which do contain nuclei and chromosomal DNA. If we could see a particular DNA sequence in these cel...

The Hypothalamus: The Body's Thermostat (Human Thermostat)

By: HWC, Views: 6975

Normal body function requires a relatively constant body temperature, which is regulated by the body's thermostat, a region of the brain called the hypothalamus. The hypothalamus generates a temperature set point for the body and appears to be the major site for the integration of temperature inf...