Mechanisms for chromosome movement Animation
Date Uploaded: 12/19/2021
Tags: Mechanisms for chromosome movement Animation mitotic metaphase spindle microtubules kinetochore microtubules kinetochores chromosome centromeres anaphase sister chromatids kinetodiore tubulin protein subunits microscopic beam ultraviolet light bleach nonkinetochore
At mitotic metaphase, the fully-formed spindle is composed of many microtubules that extend from the poles. Some of these, the kinetochore microtubules, are attached to the kinetochores of each chromosome. Kinetochores are located at the centromeres. At anaphase, sister chromatids separate and are pulled to opposite poles of the cell. During this chromosomal movement, the kinetochore microtubules become progressively shorter. The shortening occurs because the kinetodiore disassembles the microtubule into tubulin protein subunits as it passes. A motor protein actively "walks" the kinetochore along the microtubule. The tubulin subunits are reused for later microtubule assembly. The following experiment shows that the kinetodiore microtubules are shortened by disassembly rather than by contraction or movement toward the pole. Researchers used a microscopic beam of ultraviolet light to "bleach" a section of the microtubules. As the chromosomes moved toward the pole, the bleached segment did not move, indicating that the microtubules themselves do not move. More recent experiments suggest that kinetochore microtubules may also be disassembled at the poles, contributing to their shortening. In contrast to the kinetochore microtubules, nonkinetochore microtubules of the spindle (those not attached to chromosomes) do move, causing the spindle to lengthen and pushing the poles apart. The lengthening of the spindle contributes to the movement of chromosomes away from each other. In the zone where microtubules from different poles overlap, they are pushed apart by motor proteins "walking" between adjacent microtubules.
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