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DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7044

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Mechanism of enzyme action

By: HWC, Views: 7595

■ The substrate makes contact with the active site. ■ A change in the shape of the enzyme occurs resulting in the transformation of the substrate. ■ A substrate may undergo decomposition, have its atoms rearranged, or two substrates may be synthesized. ■ The products of the reaction...

What are the Parts of a Plant Cell?

By: HWC, Views: 6775

Every chloroplast in a plant cell is packed with stacks of flattened sacs called thylakoids. The thylakoid membranes contain chlorophyll, as well as most of the other components required for the light reactions of photosynthesis. The chlorophyll-containing structures within the membranes are c...

The life cycle of protein

By: HWC, Views: 7353

The life cycle of a typical protein begins with its synthesis on a ribosome. As the polypeptide chain grows, molecules of a chaperone protein bind along its length. This prevents misfolding of the nascent polypeptide. ATP binding causes chaperone release. For most proteins, the polypeptide th...

Alternative Energy Sources

By: HWC, Views: 1966

Points at which organic compounds enter the reaction stages of aerobic respiration. Complex carbohydrates are broken down into simple sugars, such as glucose. They become the substrates for glycolysis. If your body doesn't need to burn glucose for energy, glucose-6-phosphate can be co...

Glycolysis - Introduction to ATP and the burning of sugar

By: HWC, Views: 7844

Do you use sugar with your coffee or tea? Or do you occasionally drink a sport or soft drink? As millions of people do each day, they obtain energy from the sugar added or contained in these drinks. How can we understand this concept of energy within a sugar molecule? Let's take a tablespoon ...

The Krebs Cycle Animation

By: HWC, Views: 1928

The second-stage reactions of aerobic respiration. The second-stage reactions occur in a mitochondrion's inner compartment. In the first preparatory reaction, a carbon atom is stripped from pyruvate and released as carbon dioxide. The remaining carbons combine with coenzyme A and give ...

Fermentation - When Oxygen Is Absent, Pyruvate to Lactate & Pyruvate to Ethanol

By: HWC, Views: 7281

Pyruvate is the end product of glycolysis. If oxygen is present, pyruvate enters the mitochondrion where further energy yielding reactions of the Krebs cycle will take place. However, if oxygen is not present, pyruvate will enter a pathway called fermentation. This pathway regenerates NAD+ fro...

ETC Protein Complexes & Chemiosmosis (Total ATP Production and ATP Synthase)

By: HWC, Views: 7422

You will notice that FADH2 donates two electrons further downstream than NADH. This results in only two protons being pumped across the inner membrane. The final electron acceptor for these transported electrons is oxygen. Oxygen receives these electrons, plus protons from the aqueous matrix. ...