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The Lagging Strand in DNA Replication and Replication in Action

By: HWC, Views: 7238

The lagging strand is the strand of nascent DNA whose direction of synthesis is opposite to the direction of the growing replication fork. DNA backbones run in opposite directions, the strands in a DNA molecule are oriented antiparallel to one another. New DNA is made by enzymes called DNA...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 7067

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

Mechanism of enzyme action

By: HWC, Views: 7614

■ The substrate makes contact with the active site. ■ A change in the shape of the enzyme occurs resulting in the transformation of the substrate. ■ A substrate may undergo decomposition, have its atoms rearranged, or two substrates may be synthesized. ■ The products of the reaction...

Oxygen transport - methods and oxyhemoglobin

By: HWC, Views: 7568

• The blood is the medium used for gas transport throughout the body. • Oxygen is only available in the lungs. Because the partial pressure of oxygen is higher in the alveoli than in the blood, oxygen diffuses into the blood and is transported to systemic cells. • At the tissues the par...

New DNA Strand Replication

By: HWC, Views: 7146

First step: strands are separated • Helicase unwinds the DNA double helix at the replication fork • SSBs coat the single strands to prevent reannealing • Polymerase attaches nucleotide at 3' end • Synthesis is in 5' to 3' direction DNA Polymerase: • Only extends nucleic ac...

Alternative Energy Sources

By: HWC, Views: 1986

Points at which organic compounds enter the reaction stages of aerobic respiration. Complex carbohydrates are broken down into simple sugars, such as glucose. They become the substrates for glycolysis. If your body doesn't need to burn glucose for energy, glucose-6-phosphate can be co...

Glycolysis - Introduction to ATP and the burning of sugar

By: HWC, Views: 7864

Do you use sugar with your coffee or tea? Or do you occasionally drink a sport or soft drink? As millions of people do each day, they obtain energy from the sugar added or contained in these drinks. How can we understand this concept of energy within a sugar molecule? Let's take a tablespoon ...

The Krebs Cycle Animation

By: HWC, Views: 1944

The second-stage reactions of aerobic respiration. The second-stage reactions occur in a mitochondrion's inner compartment. In the first preparatory reaction, a carbon atom is stripped from pyruvate and released as carbon dioxide. The remaining carbons combine with coenzyme A and give ...

Fermentation - When Oxygen Is Absent, Pyruvate to Lactate & Pyruvate to Ethanol

By: HWC, Views: 7305

Pyruvate is the end product of glycolysis. If oxygen is present, pyruvate enters the mitochondrion where further energy yielding reactions of the Krebs cycle will take place. However, if oxygen is not present, pyruvate will enter a pathway called fermentation. This pathway regenerates NAD+ fro...