Virtual Enzyme Kinetics & Lineweaver Burk Plot




• The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). • This plot is a useful way to determined different inhibitors such as competitive, uncompetitive, and noncompetitive. • Chemical kinetics and enzyme kinetics are related, but enzyme kinetics necessitates different treatment because of the substrate's complex with enzyme catalyst. • Michaelis Menten kinetics describes enzyme-catalyzed reactions. • Vmax is the maximum velocity at saturating [S]. • KM determines shape of Michaelis Menten hyperbolic curve. • Data from kinetic experiments can be plotted as a Michaelis-Menten curve or as a line in a double-reciprocal plot. The resulting graphs can be interpreted to obtain Vmax and Km.



Copy and paste this code into your website or blog.

Add To

You must login to add videos to your playlists.


0 Comments total

to post comments.

No comments have been posted for this video yet.