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SNP Polymorphysim Microarray Chip - How to Test a Person's DNA

By: HWC, Views: 5964

To test a person's DNA, a researcher first needs a source of tissue. Most of the cells in a blood sample are red blood cells, which lack nuclei, but there are also a number of white blood cells, which do contain nuclei and chromosomal DNA. If we could see a particular DNA sequence in these cel...

Glycolysis - Introduction to ATP and the burning of sugar

By: HWC, Views: 6760

Do you use sugar with your coffee or tea? Or do you occasionally drink a sport or soft drink? As millions of people do each day, they obtain energy from the sugar added or contained in these drinks. How can we understand this concept of energy within a sugar molecule? Let's take a tablespoon ...

Nucleic acid digestion - brush border enzymes, end products & transport mechanism

By: HWC, Views: 6447

• Further digestion occurs at the microvilli (brush border) of the epithelial cells of the villi in the small intestine. • Two brush border enzymes complete nucleic acid digestion: • Phosphatases, which catalyze the cleavage of a phosphate to form a nucleoside (nitrogenous base and pent...

DNA Sequences - Dideoxy Sequencing

By: HWC, Views: 5988

A short, radiolabeled primer is annealed to the single-stranded DNA to be sequenced. The DNA serves as a template for in vitro DNA synthesis. The DNA-primer mixture is split into four separate tubes. DNA polymerase and a solution of dNTPs are added to each tube. One of the four 2',3' dideoxy-N...

ribosome

By: HWC, Views: 6336

The trp operon in E. coli contains five structural genes corresponding to enzymes that convert chorismate into tryptophan. The trpE gene contains a 5' untranslated region that plays an important role in the regulatory mechanism called attenuation. The 5' UTR contains four regions. Region 1 en...

Acid-base imbalances - metabolic acidosis and alkalosis

By: HWC, Views: 6711

• Metabolic acidosis typically results from very low HCO3- ion blood concentrations. • Metabolic alkalosis typically results from very high HCO3- ion blood concentrations.

Coaductile pathway, Timing of contraction signal & Conduction system and ECG

By: HWC, Views: 6889

• When the system is healthy, the signal to contract the entire conduction system originates in the SA node - known as the heart's pacemaker. • The SA node triggers contraction because it depolarizes at a faster rate than other parts of the conduction system. • The wave of excitation fr...

Acid-base imbalances - respiratory acidosis and alkalosis

By: HWC, Views: 6919

• The individual mechanisms involved in regulating pH work in concert with each other to ensure that blood pH is maintained within the normal range. • The two categories of pH imbalance are: • Respiratory • Metabolic • Both types of imbalances can cause either acidosis or alka...

Acid-base imbalances - compensation of metabolic acidosis and alkalosis

By: HWC, Views: 6946

1. Metabolic acidosis: • Few HC03- ions are present so blood pH becomes acidic. • Compensation is increased respiratory rate and more CO2 is removed from the blood. • Blood pH returns to normal. 2. Metabolic alkalosis: • Many HC03- ions are present so blood pH becomes alkaline...